Development of multiple wavelength simultaneous multi-level magnification SPIM (mλ-sMx-SPIM) for biological applications

It is a promising technology that can be used to generate multidimensional images, of larger biological specimens non-destructively with highly reduced photobleaching and phototoxicity. Conventional microscopic techniques rely on slicing of thick samples and hence the study of live biological processes become impossible. On the other hand, SPIM does optical sectioning using thin sheet of light.

This microscopy system can provide image resolution and/or magnification at different levels (with separate fields of view associated with the individual magnification powers) simultaneously, with all other advantages of a SPIM imaging system. This enables the biologists to study a system in its cellular level and in the whole embryonic level simultaneously with different magnification.

Multispectral imaging is a powerful method to image several target molecules together. Multispectral imaging helps to identify several target molecules simultaneously at once, thereby retrieving information from the entire volume of the sample.